STATES OF MATTER
material library & DIY tools
FABRICADEMY 2021 / 2022
STEPS
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I. Wool pre-treatment
We use acetone or ethanol to clean all dirty parts of wool fleece and remove first layers of wool fiber (cortex & cuticle) and lanolin. Then we are able to more easily open the fiber bonds.
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II. Chemical dissolution
The wool fiber will disappear in chemical solution with urea and NaOH. That means its additional layers of wool fiber will be destroyed. You will achieve a liquid yellow/brown solution which you will need to clean. Keratin is now still mixed with the rest of the chemicals.
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III. Filtration & Centrifugation
Filter and purify the extracted solution. Centrifugation serves as a tool to separate chemical molecules from molecules of wool protein. The DIY centrifuge is made of an electric drill and 3D printed parts.
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IV. Biuret test
Protein presence test with home-made Rochelle crystals.
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V. Dialysis
Instead of cellulosic dialysis tubes we will use a cow stomach to filtrate the extracted solution from the rest of the chemicals.
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VI. Freeze-drying
DIY Freeze dryer in YOUR LAB SOON!